IndieBB - Technical FAQ

As I am redesigning the main pitch-page for IndieBB in order to appeal more to the currently-untechnical audience, what little technical detail I’ve reserved for the main page will have to be stripped out; I’m taking this opportunity to write up a “Frequently Asked Questions” post to address the more technical queries I’m seeing in the survey, tweets, comments and other correspondence. I will soon also post a less technical FAQ, but given that “Less Technical FAQ” is now the design goal for the main IndieBB crowdfunding page, I’m considering it a lower priority.

What Will Your IndieBB Be?

IndieBB stands for “Indie Biotech Backbone”. When I started this blog, I had intended “Indie Biotech” to be a phrase that could be generally used, not a “trademark” for my own work, yet people sometimes still refer to my project/company as “Indie Biotech” (I do have a company for this, and it’s called “Glowbiotics”, not “Indie Biotech”!). With the same spirit in mind, I named my plasmid backbone “Indie Biotech Backbone” because I wanted it to be something that could be used by “indie” genetic engineers worldwide to make their own stuff.

Codon Optimisation: A Hidden Process Behind IndieBB

I’ve posted twice recently after a prolonged blogging absence, and here I am again. Perhaps I should always be running a crowdfunding campaign, so that I have a stake in blogging; I’d be far more prolific! I’m too principled (or, to some “dogmatic”) to include advertisement on this or any blog, and flattr revenue is far too thin to encourage more than the occasional post otherwise. In any case, today I’d like to share something with you all that I’ve been meaning to write up for ages anyway, but which becomes especially relevant in light of my IndieBB DIYbio/Biohacking/Teaching plasmid design project.

A Primer on Plasmid Design (For IndieBB)

So, in the preceding blogpost I introduced my current crowdfunding project, IndieBB: ..If you navigate to the “updates” panel on that crowdfunding page, I’ve been busy keeping things current. Among the promises I’ve made lately was to run through my workflow for total-plasmid-design as I plan to apply to IndieBB. So, here we go. Before clicking “more”, be aware that you do not need to understand any of the below to complete the IndieBB kit.

IndieBB: Open Source Biotech Starter Kit for Everyone

So I started a crowdfunding campaign today, and I’m really happy with the response and enthusiasm so far. I should probably have had a draft blog-post lined up prior to the launch, but my laptop took a dive and I suffered broken-screen-syndrome for 2.5 hours, so things were rushed. The video in the crowdfunding page on IndieGoGo and embedded here says it all; I want to create a kit that will let everyone get involved in genetic engineering, and from there to help democratise synthetic biology so we can all have a chance to shape the future of technology.

Technology Review Article & Attendant FAQ

An article covering my work was published today in the online edition of MIT Technology Review Magazine. It was written by Antonio Regalado, and he was very thorough in his research; nobody else to date has asked for scanned documentation to back up my claim of winning a Class 1 License from the EPA! The article was well written and had a great narrative flow, but some readers may be left with questions on the “crunchy bits”: details that don’t make a great story, but might satisfy a sufficiently piqued interest.